Objective
The aim of this project is to set up a novel combined wide field, confocal laser-scanning and near-field microscope for fluorescence studies of biological systems. The setup will allow to monitor the distribution of proteins in biological samples by fluorescence microscopy and to identify and localize protein interactions by means of fluorescence resonance energy transfer. Both methods will benefit substantially from the complementary techniques of confocal laser-scanning microscopy (CLSM) and scanning near-field optical microscopy (SNOM). While CLSM allows to create almost arbitrary crossections from the fluorescence distribution in a specimen, SNOM is extremely sensitive to processes on the sample surface and allows to achieve a spatial resolution of below 100 nm. The feasibility of the setup for the study of protein distributions and protein-protein interactions will be demonstrated exemplarily in an investigation of the interaction mechanisms of the HIV-1 transactivator protein Tat.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences physical sciences optics microscopy
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
50019 FIRENZE
Italy
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.