High-resolution fluorescence imaging of biomolecules

Informazioni relative al progetto

ID dell’accordo di sovvenzione: HPMF-CT-2002-01649

Progetto chiuso

Data di avvio 18 Febbraio 2002

Data di completamento 17 Febbraio 2003

Finanziato da

• Programme for research, technological development and demonstration on "Improving the human research potential and the socio-economic knowledge base" (1998-2002)

Costo totale € 65 956,00

Contributo UE € 65 956,00

Coordinato da

EUROPEAN LABORATORY FOR NON-LINEAR SPECTROSCOPY
Italia

Obiettivo

The aim of this project is to set up a novel combined wide field, confocal laser-scanning and near-field microscope for fluorescence studies of biological systems. The setup will allow to monitor the distribution of proteins in biological samples by fluorescence microscopy and to identify and localize protein interactions by means of fluorescence resonance energy transfer. Both methods will benefit substantially from the complementary techniques of confocal laser-scanning microscopy (CLSM) and scanning near-field optical microscopy (SNOM). While CLSM allows to create almost arbitrary crossections from the fluorescence distribution in a specimen, SNOM is extremely sensitive to processes on the sample surface and allows to achieve a spatial resolution of below 100 nm. The feasibility of the setup for the study of protein distributions and protein-protein interactions will be demonstrated exemplarily in an investigation of the interaction mechanisms of the HIV-1 transactivator protein Tat.

Campo scientifico (EuroSciVoc)

CORDIS classifica i progetti con EuroSciVoc, una tassonomia multilingue dei campi scientifici, attraverso un processo semi-automatico basato su tecniche NLP. Cfr.: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.

• scienze naturali › scienze biologiche › biochimica › biomolecole › proteine › proteomica
• scienze naturali › scienze fisiche › ottica › microscopia

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Programma(i)

• FP5-HUMAN POTENTIAL - Programme for research, technological development and demonstration on "Improving the human research potential and the socio-economic knowledge base" (1998-2002)

Argomento(i)

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Meccanismo di finanziamento

RGI -

Coordinatore

EUROPEAN LABORATORY FOR NON-LINEAR SPECTROSCOPY

• Contributo UE: Nessun dato
• Indirizzo: Via Nello Carrara, 1 ; 50019 FIRENZE ; Italia
• Costo totale: Nessun dato