Objective
i. Characterisation of known amine oxidases: in order to improve the activity of the A. niger amine oxidase, the gene has been subcloned resulting in approximately 3 fold greater levels of expression. The enzyme has been partially purified and Turner and coworkers are trying to characterize this amine oxidize with respect to substate specificity and stereo selectivity using different substrates. We shall begin by completing this characterization in order to asses the properties of the enzyme and provide a basis for the in vitro evolution/ selection methods detailed below.
ii. Directed evolution of amino acid/ amine oxidizes coupled with high-throughput screening: a major part of the project will be directed towards the generation of libraries of mutant amino acid and amine oxidizes followed by high throughput screening to identify enantioselective amine oxidizes for use in the deracemisation reactions. The similarity in apparent mechanism of action between amino acid oxidizes and type II amine oxidizes suggest that it should be possible to evolve one activity from the other.
iii. Use of amine oxidizes to the deracemisation of racemic chiral amines: moreover the screening/ directed evolution work it will be necessary to determine substate specificity and stereo selectivity of the new amine oxidizes that could be discovered. New enzymes will be characterized against a defined set of substrates in order to generate a range of amine oxidizes with complementary specificities.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences organic chemistry amines
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Topic(s)
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
EH 3JJ EDINBURGH
United Kingdom
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