Amine oxidases in the deracemisation of racemic chiral amines

Project Information

Grant agreement ID: HPMF-CT-2002-01669

Project closed

Start date 1 March 2002

End date 27 February 2004

Funded under

• Programme for research, technological development and demonstration on "Improving the human research potential and the socio-economic knowledge base" (1998-2002)

Total cost € 114 272,00

EU contribution € 114 272,00

Coordinated by

UNIVERSITY OF EDINBURGH
United Kingdom

Objective

i. Characterisation of known amine oxidases: in order to improve the activity of the A. niger amine oxidase, the gene has been subcloned resulting in approximately 3 fold greater levels of expression. The enzyme has been partially purified and Turner and coworkers are trying to characterize this amine oxidize with respect to substate specificity and stereo selectivity using different substrates. We shall begin by completing this characterization in order to asses the properties of the enzyme and provide a basis for the in vitro evolution/ selection methods detailed below.
ii. Directed evolution of amino acid/ amine oxidizes coupled with high-throughput screening: a major part of the project will be directed towards the generation of libraries of mutant amino acid and amine oxidizes followed by high throughput screening to identify enantioselective amine oxidizes for use in the deracemisation reactions. The similarity in apparent mechanism of action between amino acid oxidizes and type II amine oxidizes suggest that it should be possible to evolve one activity from the other.
iii. Use of amine oxidizes to the deracemisation of racemic chiral amines: moreover the screening/ directed evolution work it will be necessary to determine substate specificity and stereo selectivity of the new amine oxidizes that could be discovered. New enzymes will be characterized against a defined set of substrates in order to generate a range of amine oxidizes with complementary specificities.

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.

• natural sciences › chemical sciences › organic chemistry › amines
• natural sciences › biological sciences › biochemistry › biomolecules › proteins › enzymes

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Programme(s)

• FP5-HUMAN POTENTIAL - Programme for research, technological development and demonstration on "Improving the human research potential and the socio-economic knowledge base" (1998-2002)

Topic(s)

Data not available

Call for proposal

Data not available

Funding Scheme

RGI - Research grants (individual fellowships)

Coordinator

UNIVERSITY OF EDINBURGH

• EU contribution: No data
• Address: West Mains Road, Kings Buildings ; EH 3JJ EDINBURGH ; United Kingdom
• Total cost: No data