Objective
I recently identified all the protein components of the Drosophila PRC1 complex, establishing an unexpected connection between Polycomb group (PcG) proteins (which are involved in maintaining the repressed state of the homeotic genes during development) and components of the general transcription machinery. Other non-PcGproteins were also identified, including a sequence-specific DNA binding protein. These proteins are associated with the PcG proteins in PRC1 from 0-12h Drosphila embryos, which encompass a large portion of embryonic development. I propose to now study the composition of the complex at more defined stages of embryonic development, and to equate this composition to PcG activity using pre-established assays. A crucial time for the correct development of the Drosophila embryo is at approximately 5 hours, when the PcG take over the role of maintaining homeotic gene repression from factors that previously set up these patterns of expression. How the PcG recognize the genes it needs to maintain in a repressed state at this time is unknown, although is believed to occur through a network of recruitment through protein-protein interactions. Examination of the protein composition of the PRC1PcG complex purified from embryos staged at, and around, this important time in developmental should allow us to better understand how this family of proteins is able to associate with the genes they repress.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences developmental biology
- medical and health sciences clinical medicine embryology
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
13288 MARSEILLE
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.