Objective
Pathogenic negative strand RNA virus such as Ebola virus, measles virus, RSV and mumps virus derive their membrane envelope from the host cell. Infected cells produce new virus particles, which are thought to assemble and bud off the plasma membrane. Viral matrix proteins have been shown to be instrumental in this process and it is thought that multiple macromolecular interactions between the matrix protein and membranes, the nucleoprotein, which packages the RNA genome into ribo-nucleoprotein particles (RNP) and the cytoplasmic tails of viral glycoproteins act together to drive assembly and budding. There is also amounting evidence that enveloped viruses such as filo viruses (Ebola) and retroviruses (HIV) utilize the cellular protein machinery implicated in the formation of, multivesicular bodies, a process, which resembles virus budding morphologically. The aim of the proposal described here is to better understand the structural requirements of virus assembly, which may ultimately lead to the identification of new targets for anti-viral agents. We propose to solve the three dimensional structures of two Ebola virus matrix protein complexes:
(i)the matrix protein in complex with the cellular budding factor Tsg 101 and
(ii) the matrix protein in complex with a fragment of the nucleoprotein. The elucidation of the molecular interactions will give insight into the assembly and budding mechanism of other pathogenic negative strand RNA viruses. The applicant will enlarge and strengthen her background in protein chemistry. This comprises general protein chemistry techniques, including expression studies protein complex formation and characterization; important skills, which are instrumental for a scientist in the post genomic, are. Furthermore, the applicant will have the opportunity to learn protein crystallography and acquire a strong and solid expertise in the field of structural biology. In return, the applicants experience in molecular biology will benefit all other members of the host laboratory.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology virology
- medical and health sciences health sciences infectious diseases RNA viruses ebola
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences health sciences infectious diseases RNA viruses HIV
- natural sciences biological sciences genetics RNA
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
38042 GRENOBLE
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.