Objective
The activation of T lymphocytes is governed by the interaction of specific receptor/ligand pairs on T cell and antigen presenting cell (APC), respectively. The engagement of the T cell receptor (TCR) by peptide/MHC complexes on the APC and the interaction ofCD80/CD86 with their costimulatory or inhibitory receptors CD28 orCTLA-4 on the T cell are among the most important. Combinations of the signals are integrated by the T lymphocyte to mount a distinctive response, such as differentiation, proliferation, anergy, or cell death. T cell stimulation induces the formation of signaling protein complexes in the T cell plasma membrane domain in contact with the APC. This study seeks to investigate the effects of modulated T cell activation on the composition of these T cell signalling complexes and to correlate them with functional parameters of T cell activation. In the presence or absence of costimulatory molecules, various peptide/MHC complexes will be coupled to magnetic beads and used for activation of transgenic murine primary T cells. The formation and composition of these signaling complexes will be studied biochemically and by imaging techniques. In this project, I will enter the field of signal transduction. By working on the project, I will complement my knowledge of cell biological, immunological and biochemical methods with state of the art proteomic analysis and analysis of lipid membranes. The host, Thomas Harder, has developed a novel approach for immunoisolationg TCR-containig membrane domains and associated proteins, which will contribute to the understanding of the integration of TCR-mediated and costimulating signals at the level of signaling complexes and might very well lead to the identification of new components of TCR signalling assemblies. In this project, I will apply this method to the activation of primary T cells isolated from TCR transgenic mice. In my PhD studies, I have extensively studied the activation of murine primary T cells and their effector functions, so the areas of expertise of Thomas Harder and me optimally complement each other.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences cell biology cell signaling
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences biochemistry biomolecules lipids
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
OX1 3RE OXFORD
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.