Objective
Aspergillus niger is the main microorganism used for enzyme production within DSM Food Specialties. The research proposal aims at the utilization of modern genomics technologies in order to rationalize strain and process improvement programs for enzyme production using A. niger as a host. Since DSM has sequenced the complete genome of A. niger, DNA micro arrays and proteomics will be used for studying the expression profile of all genes for various strains under various controlled fermentation conditions. Focus will be on identification of genes involved in heterologous gene expression and genes influencing fungal morphology. Knowledge on the (differential) expression of these genes will be used to construct new A. strains by over expression / deletion of specific genes or by Classical Strain Improvement (CSI) followed by targeted screening. Alternatively fermentation conditions will be adapted based on knowledge obtained after expression profiling. These experiments should give us information on bottlenecks in (heterologous) gene expression and genes involved in viscosity /morphology with the final goal to increase the yields in A niger fermentations. Strains expressing homologous genes and strains expressing heterologous genes will be compared. Low and high copy number strains will be examined in order to identify limiting factors at high copy numbers. Mutants obtained after CSI will be compared with parent strains in order to find new leads for strain and process improvement.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences genetics genomes
- natural sciences biological sciences microbiology
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- engineering and technology industrial biotechnology bioprocessing technologies fermentation
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
2613 ax DELFT
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.