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Nmr tools for the study of transient intermediates in biomolecular processes

Objective

Objectives: The proposal aims at the development of dedicated NMR probes and the accompanying NMR tools for the structure determination of rapidity formed, short-lived intermediates that are important in biological processes. These transient intermediates can be induced in a biomolecular system by photochemical means or by a rapid change of solvent conditions. Therefore, both a rapid mixing and a laser coupled probe will be made in which all modern multidimensional NMR experiments including the use of pulsed field gradients are possible, with as little compromise on the NMR performance as possible. These NMR probes and the set of developed experimental methods will be validated for photobiology, in protein and RNA folding studies, and in CIDNP experiments.

Results: Project Manager: Robert Kaptein NMR Spectroscopy, Bijvoet Center for Biomolecular Research Padualaan 8 Utrecht 3584-CH The Netherlands Tel: 31-30 2533787 Fax: 31-30 2537623 E-Mail: kaptein@nmr.chem.uu.nl

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

UTRECHT UNIVERSITY
Address
8,Padualaan 8
3584 CH Utrecht
Netherlands

Participants (3)

CONSORZIO INTERUNIVERSITARIO RISONANZE MAGNETICHE DI METALLOPROTEINE PARAMAGNETICHE
Italy
Address
Via Luigi Sacconi ,6
50019 Sesto Fiorentino
JOHANN WOLFGANG GOETHE UNIVERSITAET FRANKFURT
Germany
Address
9,Marie-curie-strasse 9
60439 Frankfurt-am-main
THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
United Kingdom
Address
South Parks Road
OX1 3QZ Oxford