Our project is devided in five different Tasks :
Task 1 Hapten synthesis
Task 2 Hapten conjugation to proteins (preparation of immunogen), hapten conjugation to fluorescent dye molecules.
Task 3 Immunization.
Task 4 Antibody characterization and optimization of assay conditions .
Task 5 Assay validation.
For each compound we will follow these five Tasks.
We will look for industrial exploitation in case of successful development of the assay.
Many hundreds of pesticides are widely used in Europe. This may pose problems for possible contamination of waters, food and other matrices.
At present they are mostly determined in laboratory by instrumental methods involving liquid or gas chromatography. These techniques are, however, time consuming, expensive, and require skilled personnel.
Immunoanalytical methods for the detection of small molecules have recently gained importance in environmental monitoring. Immunochemistry promises to dramatically reduce the cost of residue analysis of certain toxic substances in the environment while retaining the requisite sensitivity, precision and accuracy.
In this research proposal we suggest to develop enzyme-linked immunoassay (ELISA) and polarization fluoroimmunoassay (PFIA) systems for the detection and separation of candidate pesticidal compounds. In parallel to these assays and in order to confirm the above immunoanalytical systems, HPLC and GC-MS validation experiments are also planned. Interlaboratory analysis will be done, comparing performances (such as LOD, linearity, range, etc.) and results with different techniques.
The present proposal focuses on selected pesticides chosen on the basis of these criteria:
- They are widely used particulary in CEC/NIS countries, but also in EU countries.
- They are difficult to detect or monitor on the routine basis.
- There is no rapid assay commercially available for them.
Target pesticides include myclobutanil, fenoxycarb, propanil, 2,4,5-T, mecoprop, acetochlor and some degradation products.
Funding SchemeCSC - Cost-sharing contracts
119 899 Moscow