Objective
The main objectives are as follows:
* To conduct field studies of FBNYV build-up and spread in Egypt and Syria to obtain a better understanding of the factors involved in virus survival and spread under field conditions.
* To develop screening methods which will permit the precise determination of virus resistance levels in the existing faba bean, chickpea and lentil germplasm.
* To determine the incidence, distribution and variability of FBNYV in countries of West Asia and North Africa (WANA) by providing diagnostic tools to collaborators of the existing ICARDA network.
* To characterize the FBNYV genome by infectivity tests, by determining FBNYV gene functions and variability as well as by testing in vitro engineered replicase-associated protein (Rep) variants of FBNYV in a single cell-based replication assay for their capacity to inhibit the replication of wild-type FBNYV.
* To use in vitro engineered Rep genes for generating transgenic faba bean plants and determine the levels of virus resistance in these lines.
Expected Outcome
The work carried out in this project should provide an updated overview on the incidence, distribution and variability of FBNYV in WANA countries and give a better understanding of FBNYV ecology and epidemiology. This information will be essential for devising agricultural practices for FBNYV control. Improvement of the screening methodology and evaluation of FBNYV resistance in available germplasm will lead to the use of host resistance to FBNYV as another means of conventional control. The analysis of the FBNYV gene functions and variability and the design of defective interfering Rep protein variants will hopefully lead to the generation of transgenic faba bean plants with high levels of FBNYV resistance. The project will thus contribute to sustainable agricultural production in countries of West Asia and North Africa (WANA) through appropriate use of agricultural practices, resistant genotypes and transgenic plants.
The key activities envisaged are:
* Epidemiological and ecological studies to obtain a better understanding of the incidence, distribution and variability of FBNYV in WANA countries and the factors involved in FBNYV epidemics under field conditions.
* Development of improved screening methods for locating and evaluating virus resistance in the existing faba bean, chickpea and lentil germplasm under greenhouse and field conditions.
* Following analysis of the functions and variability of the FBNYV genes, infectivity tests with purified FBNYV particles and various combinations of cloned viral DNA components will be conducted using biolistic bombardment of faba bean seedlings. A system for FBNYV replication in suspension culture cells will be established for testing defective interfering Rep protein functions.
* Gene cassettes suitable for the transformation of faba bean will be constructed, and transformed faba bean lines will be generated and evaluated.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology virology
- medical and health sciences health sciences public health epidemiology
- natural sciences biological sciences ecology
- natural sciences biological sciences biochemistry biomolecules proteins
- agricultural sciences agriculture, forestry, and fisheries agriculture grains and oilseeds legumes
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
14195 BERLIN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.