Objective
The crown gall is a plant disease caused by bacteria belonging to the genus Agrobacterium. It is presently the main bacterial disease of crops produced in nurseries in Mediterranean countries. The long term objective of the project is to decrease the prevalence of crown gall in nurseries to below a reasonable threshold providing substantial incomes to growers. This objective will be achieved by the development of new sanitary practices based on epidemiology, biocontrol and genetic control. As a specific objective, the scientists plan to establish a common set of standardized procedures to detect, identify and monitor the fate of the causative agent and to critically view the methods that could be used to control crown gall disease.
Expected Outcome
The work carried out in this project should provide :
1. The most important collection of Agrobacterium isolated in standardised conditions in six countries around the Mediterranean Sea; this will furnish useful data about the epidemiology of Agrobacterium as well as strains belonging to new types of agrobacteria and pTi.
2. Tools for phytosanitary inspection procedures to lower pathogenic populations will be evaluated and delivered.
3. Extended and integrated use of efficient biocontrol agents on major crops as well as resistance assessment of commercially important material.
The key activities envisaged are :
* Isolation, identification, detection and quantification of numerous pathogenic agrobacteria in various crops to study the origin and fate of agrobacterial populations within and between different crops and countries.
* Accurate characterisation of bacterial strains by PCR, RFLP, AFLP, sequencing and DNA hybridization will be conducted at both chromosomal and pTi levels by using ribosomal and vir and T-DNA regions respectively. This should lead to the discovery of new agrobacteria and pTi encoding for the biosynthesis of new opine compounds. Persistence of agrobacteria in plants and soil, including study of the stability and dispersion of the pTi, will be experimentally evaluated.
* Evaluation of procedures (sanitary practices, solarisation, heat treatments, etc.) to lower pathogenic populations in plant and soil reservoirs.
* Development of biological, genetical and physiological controls, including : testing efficiency of available biocontrol agents (K84), method to screen biocontrol efficiency in plant, and screening of new biocontrol agents; searching for intrinsic resistance among cultivars; and evaluating the control efficiency of compounds extracted from weeds.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences genetics DNA
- medical and health sciences health sciences public health epidemiology
- agricultural sciences agriculture, forestry, and fisheries agriculture
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
69622 Villeurbanne
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.