Development of new immunoanalytical systems for pesticides monitoring on the base of microformat imaging and acoustometric techniques

Información del proyecto

Identificador del acuerdo de subvención: INTAS-1999-01461

Proyecto cerrado

Fecha de inicio 1 Junio 2000

Fecha de finalización 30 Noviembre 2002

Financiado con arreglo a

• International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993-

Coste total

Sin datos

Aportación de la UE

€ 57 500,00

Coordinado por

Lund University
Sweden

Objetivo

Due to wide application of herbicides in agriculture a task of their control in environment and food product became to be extremely important nowadays. The main demands for modern analytical techniques are their specificity, sensitivity and rapidity.
In frame of the project new immunoanalytical techniques will be proposed for express immunodetection of pesticides from two chemical classes - chlorinated phenols (2,4-dichlorophenoxyacetic, 2,4,5-trichlorophenoxyacetic, and 2-chloro-4-methylphenoxyacetic acids) and derivatives of s-triazine (simazine, propazine, and atrazine). Two approaches will be used in these assays.
The first one is based on measurement of ultrasonic waves spreading in the solution of immunoreactants. Techniques of direct monitoring of the immune binding and indirect ones (with the use of enzyme labels and enzyme-loaded liposomes) will be characterised. An original portable device for sensitive measurements of ultrasonic velocity and absorption in submicroliter volumes of liquids will be used for these analyses. Different pairs of enzymes and substrates will be compared in connection with their applicability for the acoustic immunoassay.
The second approach employs a microformat assay of the immune complex immobilised on a thick-film glass slide with chemiluminescent detection. Use of a charge-coupled camera in this assay permits integration of fluctuating fluorescent signal over time, thus increasing sensitivity of the measurements.
The proposed new techniques will permit rapid and sensitive monitoring of pesticide pollution. As a last step of the project, the developed techniques will be applied to testing of different environmental samples (water, soil) and agricultural products. Main metrological parameters of the assays, such as their sensitivity, duration, accuracy, precision, reproducibility, will be determined. Methodological guide for pesticides detection by the proposed techniques will be prepared.
Criteria of the project successful solving will be the development of new immunoanalytical systems for pesticides detection with the following properties: working range of detection - 0.1-0.5ng/mL assay duration - 10-15 min for field tests and no more that 1 h for stationary studies, revealing of no less that 90% of pesticide molecules in water, soil, and food samples.
The developed approaches will have significance for rapid diagnostics of not only different pesticides, but other biologically active compounds as well.

Programa(s)

• IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993-

Tema(s)

• 5 - Earth Sciences, Environment, Energy
• OPEN - OPEN Call

Convocatoria de propuestas

Datos no disponibles

Régimen de financiación

Datos no disponibles

Coordinador

Lund University

• Aportación de la UE: Sin datos
• Dirección: Getingevagen 60 ; 221 00 Lund ; Suecia
• Coste total: Sin datos

Participantes (3)

Hebrew University

Israel

• Aportación de la UE: Sin datos
• Dirección: 91120 Jerusalem
• Coste total: Sin datos

Russian Academy of Sciences

Rusia

• Aportación de la UE: Sin datos
• Dirección: Leninsky prospect 33 ; 117071 Moscow
• Coste total: Sin datos

Samarkand Medical Institute

Uzbekistán

• Aportación de la UE: Sin datos
• Dirección: Timur street 14 ; Samarkand
• Coste total: Sin datos