Objective
Virus-encoded movement proteins (MP) are essential for cell-to-cell translocation of plant viral genomes. It is widely believed that intra- and intercellular trafficking of viral MP and transport RNP non-virion (MP-RNA) or virion (CP-RNA) complexes depend on their interactions with cellular receptors, the MP-binding proteins (MPBPs). A collaboration of SCRI, MSU and HU teams in frame of INTAS project (Reference number: INTAS-96-1683) resulted in identification and isolation of TMV MP binding proteins, which may mediate and regulate TMV MP and/or MP-RNA complexes intra- and intercellular transport. It was found that three tobacco leaf cell wall (CW)-associated proteins (34K, 42K and 50-55K) specifically bind TMV MP. The 34K protein was isolated by MP affinity chromatography (MPAC), microsequenced and identified as pectin methylesterase (PME), a ubiquitous plant enzyme involved in CW growth and regeneration. Presumably PME localized in plant intercellular connections, the plasmodesmata (PD), plays a role of the MP receptor. The full-length gene of PME was cloned and expressed in E. coli and antibodies to PME were obtained. These procedures will be applied to the isolation of other MPBPs from plant cells and their characterization.
Furthermore, because pathogens usually adapt the host cell pathways for their life cycle, MP-MPBP interactions most likely reflect a more general and fundamental biological phenomenon, intercellular communication and trafficking of macromolecules within and between plant cells. Presently, however, very little is known about the cellular MPBPs and their interactions with viral MPs and transport RNP complexes during macromolecular transport.
The proposed interdisciplinary research will start filling this gap by identifying and characterizing the architecture and structure of such complexes by atomic force microscopy (AFM) and Biacore system, and elucidating the changes in the structure of complexes following phosphorylation in relation to their functions.
These include:
Complexes formed by the MPs and host-plant-coded MPBPs.
Purified MPBPs, in particular, the pectin methylesterase (PME);
Complexes formed by MPBPs and "MP-RNA" ribonucleoproteins (RNPs);
Nontranslatable non-virion "MP-RNA" or virion "CP-RNA" RNP-complexes, and the same RNPs converted into a translatable form after phosphorylation of the MP (or CP);
Native and phosphorylated viral genome-linked protein (VPg) exposed on the surface of the potato virus A. A putative complex formed by the virion VPg with cellular proteins.
Comparison of architecture and structure of biomolecular complexes with different types of movement will shed more light on the mechanisms of movement and explain functional differencies between different viral MPs. Structure-function analysis of biomolecular transport complexes will provide both novel information on molecular mechanisms of movement of macromolecules and tools for further studies.
The present project is aimed at joining the efforts of the four teams (SCRI, MSU, HU, CB) specialized in virology, molecular biology and genetic engineering with the abilities of the ATC team highly experienced in AFM, for further investigation of structural and biological properties of macromolecular transport complexes.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
This project has not yet been classified with EuroSciVoc.
Be the first one to suggest relevant scientific fields and help us improve our classification service
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Data not available
Coordinator
DD2 5DA Dundee
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.