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Contenu archivé le 2022-12-23

The common shrew (Sorex araneus) of Europe and Siberia - a primary model of chromosome evolution

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Sorex araneus is a well-studied species with respect to chromosomal variation, but studies in the past have largely been concentrated in central and western Europe. Ancestrally the species had a largely acrocentric karyotype, but metacentrics have arisen by Robertsonian fusions; with different chromosome races defined by different sets of metacentrics and acrocentrics. The main concern of this research project was the collection and chromosomal characterization of S. Araneus over the whole area of the Former Soviet Union, which, though barely studied before, represents the main part of the distributional area of the species. The project was remarkably successful; 328 S. Araneus from 85 sites were karyotyped. S. araneus proved to be just as variable within the FSU as it is elsewhere, and 16 new chromosome races were described. New data on the distribution of a further 6 races were also obtained. From the distribution and relationship of these 22 races, in the context of those found in western and central Europe, inferences could be made about the colonization history of S. Araneus at the end of the last glaciation. The main chromosomal lineage of central and western Europe, viz. the 'West European Karyotypic Group' defined by metacentrics hi and gm, apparently colonized Ukraine and parts of European Russia as well as more western areas of Europe at the end of the glaciation, although the source area is still uncertain. Another lineage, defined by metacentrics ip, go and hn, apparently occupied a glacial refugium in the Ural Mountains and spread northwards to Finland at the end of the glaciation. One member of this group of races (the Novosibirsk race) spread over a huge area of western Siberia (estimated at 700,000 km2) on draining of a glacial lake. A third lineage of races, characterized by metacentric hk, has been identified in the vicinity of the Baltic States. There is evidence that the three lineages came into pairwise contact to produce hybrid races and that metacentrics have, in some instances, become modified by whole-arm reciprocal translocations. Mitochondrial DNA studies indicate that the S. Araneus currently present in the FSU derive from a recent (late Pleistocene) expansion. Thus, the origin of chromosome races within this region are unlikely to represent events earlier than the last glaciation. During the project molecular, chromosomal and biogeographical studies were carried out on four other species of shrew: Sorex tundrensis, Sorex radeii, Sorex minutus and Suncus murinus. Tissue cultures of Sorex radeii and four chromosome races of S. Araneus were cryopreserved within the collection of V. Volobouev (Museum National d'Histoire Naturelle, Paris) as a resource for the international community.

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