Objective
During the last few years great interest has been shown in the evaluation of the role of chromatin structural domains at the level of gene expression regulation. These domains are referred to as large-scale chromatin organisation and each of these domains can exhibit different levels of chromatin folding. It is now accepted that the folding/unfolding of chromatin fibre between its different levels of organisation is strictly related to the cell functioning and therefore to the gene expression regulation. These topological domains can be studied either in situ or in vitro (on isolated chromatin).
At the level of isolated chromatin this project will evaluate the length of the topological domains (by electrophoresis) and the diameter and gyration radius of the fibres forming the domain (by X-ray and light scattering). Integrative information will be obtained by means of differential scanning calorimetry and by a new biophysical tool using the circular intensity differential scattering. Information on the 3D distribution of the chromatin domains inside the nucleus will be obtained by in situ experiments through fluorescence computational optical sectioning microscopy and complementary information can be obtained by different techniques such as confocal and electron microscopy.
In connection with these structural studies it will be interesting to analyse the DNA supercoiling of the chromatin domains while, with regard to the cell function aspects, the level of gene expression will be evaluated by measuring the mRNA amount. The possible effect of transcription inactivation and long-distance interactions at the level of chromatin organisation can be analysed for the study of the structure/function relationship. At last the association between non-histonic proteins and chromatin-DNA will be observed in order to evaluate their role in maintaining the activity of some specific genome regions. DNA protein cross-linking and nuclease tests, methods of protein image and immunoaffinic isolation of DNA-specific protein complexes will be carried out for the study of the structure of functioning chromatin and the regulation of its activity.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
This project has not yet been classified with EuroSciVoc.
Be the first one to suggest relevant scientific fields and help us improve our classification service
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Data not available
Coordinator
16153 Sestri Ponete Genova
Italy
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.