Objective
The purpose of this project is a complex analysis of the molecular mechanisms underlying the regulation of neurotransmitter-activated ionic channels during ontogenetic and phylogenetic development of the nervous system. The study will be conducted on mammalian neurones in the central nervous system (hippocampal slices and tissue culture) and on invertebrate neurones (from the mollusks Aplysia and Lymnaea stagnalis).
Using a combination of patch-clamp recording techniques and laser scanning confocal microscope on neurons in hippocampal slices, the properties of ionic channels activated by glutamate and GABA during the first days of postnatal development will be investigated.
Using the calcium-sensitive fluorescent dyes (Indo-1 and Fluo-3) and the patch-clamp recording technique, simultaneous monitoring of neurotransmitter-activated ionic currents and intracellular Ca2+ concentration ([Ca2+]i in cultured hippocampal neurones as well as hippocampal slices of different ages will be conducted.
Using biochemical analysis of endogenous phosphorylation of proteins in homogenates of hippocampal slices and specific antibodies raised against synaptic proteins, the possible role of proteins specifically involved in synaptic transmission during the establishment of synaptic contacts and synaptic transmission during the maturation of the brain will be estimated.
Fields of science (EuroSciVoc)
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
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Coordinator
75674 Paris 14
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.