Objective
NAD-dependent formate dehydrogenase (EC 1.2.1.2 FDH) is of great interest for theoretical reasons and for practical applications. FDH is the best model system for the detailed investigation of the mechanism of hydride transfer. It is already used for the regeneration of NADH in coupled enzymatic reductions for the synthesis of fine chemicals.
Using methods of protein engineering the following goals for this project to be achieved: the coenzyme specificity of FDH should be changed to NADP in order to develop a NADPH regeneration system; the thermal stability of FDH should be improved along with the oxidative deactivation being minimised; with the help of the known X-ray structure of bacterial FDH mutants will be generated and characterised to elucidate the reaction mechanism of FDH; and structural studies will be made of bacterial FDH muteins as well as C. boidinii FDH to understand the structural basis for the change from a random to an ordered kinetic mechanism in the same reaction.
Fields of science (EuroSciVoc)
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Funding Scheme
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Coordinator
52404 Jülich
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.