Objective
The project is focused on in vivo translational control and its role in physiological processes in eukaryotic cells. Primary studies will be concentrated on the SUP35 omnipotent suppressor of the yeast Saccharomyces cerevisiae. Former experiments have revealed that this gene is one of the key genetic components controlling translational fidelity in yeast. Recently obtained data also show that the SUP35 gene product is probably a yeast analogue of prion proteins of higher eukaryotes. This enables a convenient model system to be developed for studying mechanisms of protein-based heredity. Finally, the SUP35 gene is of general interest since its homologues were recently found in several higher eukaryotic organisms including man.
The basis of this project is to analyze the regulation of expression of the SUP35 gene and to identify the possible function(s) of the corresponding gene product. Special attention will be given to studying the relation of the SUP35 gene product to the non-Mendelian determinant [psi], the probable analogue of prion proteins of higher eukaryotes. All these problems will be investigated through several approaches involving the specialized abilities of the different participating laboratories.
A homologous cell-free translation system that is dependent on the SUP35p protein will be generated in order to estimate its role in protein synthesis. On the other hand, the identification of genes which interact with SUP35 will be undertaken; this can be done either by searching for genes, the expression of which is under the control of the SUP35 gene product, or by identifying physically interacting components. Several efficient genetic and biochemical methods are already operational. Finally, study of the expression of SUP35 under several conditions, especially stress, will be analyzed and the components responsible for this regulation identified.
These various approaches may help in defining the inter-relationships of translation with other cellular processes in yeast.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
This project has not yet been classified with EuroSciVoc.
Be the first one to suggest relevant scientific fields and help us improve our classification service
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Data not available
Coordinator
1081 HV Amsterdam
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.