Objective
An integral approach including genetic engineering and physicochemical methods as well as theoretical analysis will be used to determine interrelationship between the bacterial ribonucleases barnase and binase structure, function and thermal stability. Molecular mechanisms of protein-protein complex stabilization will be elucidated on an example of ribonucleases interaction with the natural inhibitor barstar. The amino acid residues critical for the stability will be mapped by introduction of directed mutations into ribonucleases / barstar structure and comparative study of native and mutant forms using scanning and isothermal microcalorimetry, CD, fluorometry, X-ray, NMR and molecular modeling. Detection of denaturation peculiarities on ribonucleases from different bacterial species will allow to estimate the rate of the protein stability alteration during microevolution. Motives for structural stability and flexibility will also be searched for in RNase A and compared with the bacterial ones. As a result, a concept of directed change of ribonucleases thermal stability will be formulated.
The expected results will be presented in scientific papers published in international journals.
Fields of science (EuroSciVoc)
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
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Coordinator
3001 Heverlee - Leuven
Belgium
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.