Objective
Biodiesel/fuel is defined as fatty acid methyl or ethyl esters of vegetable oils, animal fats or their greases, when they are used as fuel in many applications. It can be produced by chemical approach, but this process has several disadvantages. Use of lipases largely prevents these disadvantages. Main advantage of using enzymatic process for biodiesel production is to use low price feedstocks, which have high content of free fatty acids. Lipases (EC 3.1.1.3) are triacylglycerolester hydrolases, which catalyse ester hydrolysis and synthesis reactions.
The main objectives of this project are to create an improved lipase, which catalysis efficiently transesterification of vegetable oils and their soapstocks and microalgal oil with alcohols and to develop a novel technology to produce high yields of biodiesel using enzymatic bioreactor. To achieve of these objectives, we aim to select a specific and active microbial lipase for hydrolysis and alcoholyses reactions, clone, express and purify the selected lipases, optimise of biodiesel production reactions for native and recombinant lipases, enhance of recombinant lipase activity and specificity by mutagenesis and immobilization, design and optimise of enzymatic bioreactor for high yield production of biodiesel.
Also, we aim to integrate scientific and technical expertise of INTAS and NIS countries in order to exchange information and technology in the most effective manner. The consortium includes 2 INTAS and 2 NIS partners. The multidisciplinary approach brings expertise from microbiology, molecular genetics, biotechnology, oil chemistry, biochemistry, chemical and biochemical engineering. All research partners are highly qualified and well experienced in their field of research and will contribute effectively for the successful completion of this project. The collaboration will also facilitate the visit of several young scientists from NIS teams to INTAS partners' laboratories to obtain the state-of-the-art training. Initially, specific and active lipase for biodiesel production from available sources will be selected. Using the best microorganisms, lipases' genes will be cloned into heterologuos host and bulk quantities of purified highly active lipases will be produced. Reaction conditions for biodiesel production by native and recombinant lipases will be optimised.
The enhancement of recombinant lipase activity and stability by mutagenesis and immobilisation in solid matrix will be carried out. Removal of glycerol as by product from reaction mixture will be developed. Optimal temperature, alcohol/oil molar ratio and operational stability of enzyme in newly designed bioreactor will be investigated. The quality of produced biodiesel will be tested. The specified goals and objectives of the Proposal will extend state-of-the-art of current knowledge in lipases, their application and will show feasibleness of biotechnological production of biodiesel as alternative fuel.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
This project has not yet been classified with EuroSciVoc.
Be the first one to suggest relevant scientific fields and help us improve our classification service
You need to log in or register to use this function
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Data not available
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Data not available
Coordinator
STUTTGART
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.