Objective
In the proposed project we intend to apply a powerful arsenal of spin chemistry techniques to study the reactivity, structural and magnetic properties of elusive radical intermediates of biologically important molecules. We plan to exploit the advantages of the Chemically Induced Dynamic Nuclear Polarization (CIDNP) and Magnetic field Affected Reaction Yield (MARY) techniques. Due to their capability of sensitively detecting short-lived radical species that are beyond the reach of the conventional EPR-spectroscopy, these techniques are suited to provide unambiguous determination of HFI constants and g-factors of the transient radicals and to allow identifying the elementary steps and mechanisms of the reactions in which they are involved.
We plan to apply these techniques in particular to studying the magnetic resonance properties and reactivity of radicals formed in reactions of amino acids and nucleotides, the intramolecular charge transfer in peptides, proteins and oligonucleotides, electron transfer reactions between the DNA bases and amino acids, and the magnetic field effects on the enzymatic phosphorylation reaction. The time-resolved version of CIDNP technique will be employed to study the spatial structure and intramolecular mobility of proteins in native, denatured and partially folded states. Polarization transfer will be studied in detail and utilized to enhance the sensitivity of NMR techniques. A key element of the project is its interdisciplinary character comprising biological, chemical, and physical approaches.
The most important results expected include:
- A detailed analysis of fast reactions involving amino acids and nucleotides, including the determination of the reaction mechanisms, the reaction rate constants, and characterization of the magnetic resonance properties of the short-lived intermediates formed in the course of these reactions.
- The characterization of the mechanisms and the kinetics of the intra-molecular charge transfer in peptides, proteins, oligonucleotides, and inter molecular electron transfer between amino acids and DNA bases that mimics the DNA repair.
- New theoretical methods, suitable for a wide range of spin chemistry applications.
- New information on the spatial structure and intramolecular mobility of proteins in different physical states.
- The determination of structural changes in a number of proteins caused by ligand and substrate binding, while the efficiency and kinetics of the binding process will be measured by utilizing polarization transfer from the protein to the ligands.
- Determination of the mechanisms of polarization transfer in low magnetic fields, exploitation of the remarkable amplitudes of the CIDNP signals to improve the notoriously low sensitivity of the NMR of biomolecules.
- The identification of the mechanisms responsible for the magnetic field influence on enzymatic reactions and the measurement of the magnetic properties of the involved radicals.
Fields of science (EuroSciVoc)
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding Scheme
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Coordinator
BERLIN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.