The proposed project wants to accomplish tile following results in sea breeding:
a) to use frozen milt of males sampled in different farms to progeny tested them;
b) to use progeny test results to assess a genetic index to each sampled mate for trait of economical interest;
c) to test the significance of G x E interaction by ranking the genetic values of the sampled males at two different location where progenies were grown.
Two large fingerlings producers are involved: each of them will sample a minimum of 10 males to be genetically evaluated. Milt will be collected from sampled males and frozen. Frozen milt will be used to fertilise two groups of females (female reference population). Progeny groups will be kept at two different locations and fattened following two different procedures. The work will be organised according to the following TASKS.
A. Sampling males from different SMEs, collecting and freezing milt.
B. Breeding the reference female populations.
C. Measuring the genetic differences among the sampled males for the collected measurements.
For taskA (sampling males collecting and freezing milt) the major deliverables will be:
- on farm validation of a protocol to freeze seabream sperm in straws;
- a frozen semen bank compounded by doses of sampled seabream sires.
For taskB (breeding females population) the major deliverable will be:
- measurements of different fertility rates for seabream frozen sperm used on-farm.
For taskC (measuring genetic differencies among sampled males) the major deliverables will be:
- a genetic comparison of participating SMEs;
- genetic indexes of sampled males (sires).
This trial will also permit to estimate genetic parameters (heritabilities and correlations) for different traits of economical interest.
Funding SchemeCRS - Cooperative research contracts
Torre Canne Di Fasano (Br)