The primary objective of this study is to develop whole animal gene expression profiles to identify candidate genes which are associated with resistance to various stressors in rainbow trout. Information obtained from genomic expression profiles of stressed fish is a necessary base for further development of a marker-assisted selection strategy.
The primary goal will be achieved by utilising DNA micro-array technology together with whole animal physiology to address several subsidiary objectives:
1-The gene expression profiles of selected tissues will be determined in fish exposed to standardised stressors.
2. Genes whose expression is linked to adaptation response to stress will be identified.
3. The difference in gene expression profiles between selected trout, which display a divergent trait (stress responsiveness, growth, salinity adaptation) will be used to identify potential candidate markers of genotypic variation in stress responsiveness.
The work programme is divided into three phases.
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