Objective
We aim to develop a novel strategy for combined gene/chemotherapy of cancer with nucleoside analogues (NA) that is superior to the existing HSV-I thymidine kinase (TK)/ganciclovir treatment of cancer. NA-metabolizing enzymes, including VZV TK, mitochondrial TK-2, the insect multifunctional deoxynucleoside kinase, nucleotides and thymidine phosphorylase, will be cloned and expressed to investigate the NA substrate affinities and to crystallize the enzymes with their preferred NA. Design of novel NA and mutated enzymes will be performed. Tumour cells will be transfected with the enzyme cDNA constructs to determine the increased cytostatic potential of the NA. The effect of targeted expression of the enzymes in either the cytosolic/nuclear or in the mitochondrial compartment of the tumour cells will be investigated. Also monocyte/macrophages as a model of resting tumour cells with low replicating capacity and low dNTP pools will be transiently transfected. Metabolism and mechanism of action studies will be performed on the NA. The most promising pairs of enzyme construct/NA will be evaluated in vivo SCID mice models to prove our concept.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences clinical medicine oncology
- natural sciences biological sciences genetics nucleotides
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
3000 LOUVAIN / LEUVEN
Belgium
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