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Content archived on 2024-05-15

Transposon-based strategies for functional genomic analyses in xenopus tropicalis, a vertebrate model system for developmental and biomedical research

Objective

The pace at which sequence information is generated in various model organisms is rapidly growing. However, the ability to generate genetic information has far outstripped our ability analyse it to the point that it is medically relevant. Clearly, functional genomic analyses might benefit from
1) novel genetic models, whose purpose is to complement existing models to obtain comprehensive genetic data on the functional level; and
2) methodology that can bridge the gap between genotype and phenotype in order to understand gene function in vertebrate organisms. Accordingly, we propose to lay the foundation of using Xenopustropicalis as a vertebrate model system for biomedical research by developing transposable elements as molecular tools for functional genomics. In this project we propose to cover the full spectrum of gene analysis by
1) developing efficient transposable element vectors for the introduction of nucleic acid into frog chromosomes;
2) applying transposon technology to efficiently inactivate and rapidly isolate genes in the amphibian model;
3) applying transpose on technology to express genes in different tissues of the amphibian model in order to establish gene function and involvement of gene products in cellular pathways.

Call for proposal

Data not available

Coordinator

MAX DELBRUCK CENTER FOR MOLECULAR MEDICINE
EU contribution
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Address
ROBERT-ROESSLE-STRASSE 10
13092 BERLIN
Germany

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Total cost
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Participants (4)