Objective
Understanding how the regulation Beta1 integrin is achieved is a very active research field. This control likely involves a direct binding of a cytoplasmic protein with the integrin. I have shown using in vitro and cell culture approach that ICAP-1 (Integrin Cytoplasmic domain Associated Protein) negatively regulates Beta1 integrin function. With my proposal I will use knockout technology to unravel ICAP-1 function during development. To achieve this goal I have joined an outstanding laboratory in this field. I will generate two different mouse strains. The first one will be a complete knockout of the gene, while the second will be an exon specific deletion within the ICAP-1 gene. The deieted exon encodes the integrin binding site, and these mice carrying such a mutation will be useful to ask whether ICAP-1 interaction is essential in vivo. Both targeting vectors have been already electroporated into ES cells. I will start the phenotype analysis of these mice next year. This will be done using cell biology, biochemistry, histology and immunochemistry. Altogether these different approaches will give a deep insight into ICAP-1 function and will provide a better understanding of the regulation of Beta1 integrin functions.
Fields of science
Call for proposal
Data not availableFunding Scheme
RGI - Research grants (individual fellowships)Coordinator
221 85 LUND
Sweden