This proposal aims at providing high performance proteome analysis by developing a fully automated system based on integrated micro fluidics. Biological fluids will be pre-fractionated by protein isoelectric points using mini-segmented focusing chambers. Proteins will then be automatically driven into a microchip to be separated according to mass and affinity in micro sieving matrices, and injected into a high resolution mass spectrometer by a microchip-nanoelectrospray interface. This platform includes innovations in prefractionation, protein micro separation, matrices, micro fluidics and MS interface; it will increase range and selectivity of proteome analysis, and improve analysis speed, sample economy and cost effectiveness. Initial applications will be assessed as model tasks. In the longer term, these technologies will also lead to improve miniaturized tools for protein-based diagnostics.
Funding SchemeCSC - Cost-sharing contracts