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New microfluidic-mass spectrometry technologies for high performance proteomics (MICROPROTEOMICS)

Objective

This proposal aims at providing high performance proteome analysis by developing a fully automated system based on integrated micro fluidics. Biological fluids will be pre-fractionated by protein isoelectric points using mini-segmented focusing chambers. Proteins will then be automatically driven into a microchip to be separated according to mass and affinity in micro sieving matrices, and injected into a high resolution mass spectrometer by a microchip-nanoelectrospray interface. This platform includes innovations in prefractionation, protein micro separation, matrices, micro fluidics and MS interface; it will increase range and selectivity of proteome analysis, and improve analysis speed, sample economy and cost effectiveness. Initial applications will be assessed as model tasks. In the longer term, these technologies will also lead to improve miniaturized tools for protein-based diagnostics.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

UNIVERSITAET KONSTANZ
Address
Universitaetsstrasse 10
78457 Konstanz
Germany

Participants (5)

ADEMTECH S.A.S. (ADVANCED EMULSION TECHNOLOGIES)
France
Address
Av. Docteur Schweitzer 162, Centre Condorcet
33600 Pessac
DIAGNOSWISS S.A.
Switzerland
Address
Route De L'ile-au-bois, C/o Cimo S.a., Case Postal
1870 Monthey
INSTITUT CURIE
France
Address
Rue Pierre Et Marie Curie 11
75231 Paris
SWISS FEDERAL INSTITUTE OF TECHNOLOGY LAUSANNE
Switzerland
Address
Epfl-dc-le
1015 Lausanne
UNIVERSITA DEGLI STUDI DI VERONA
Italy
Address
Strada Le Grazie 15
37129 Verona