Virus-induced gene silencing : unravelling the basis of a mechanism and its exploitation for the analysis of a multitude of individual gene functions in plants (VIS)

Objective

The objective of the proposal is to produce a detailed description of the molecular mechanisms underlying different stages of virus-induced gene silencing (VIGS) in plant cells. VIGS is a recently discovered sequence - specific RNA degradation mechanism in plants, that has a huge potential for the functional analysis of individual genes on the genome-wide scale, provided that its general principles are better understood. We plan to investigate the following aspects of VIGS: i) viral features detected by the plant^TMs surveillance mechanism; ii) suppressors of VIGS; iii ) host factors required for VIGS; v) role of DNA methylation in VIGS . The fulfilment of the project should pave the path for the construction of generally applicable efficient VIGS vectors for the functional analysis of multitude of plant genes.

Programme(s)

• FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002

Topic(s)

• 1.1.1.-8. - Research into genomes and diseases of genetic origin

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

NATIONAL INSTITUTE OF CHEMICAL PHYSICS AND BIOPHYSICS

Address

23,Akademia Tee 23
12618 Tallinn
Estonia

Participants (7)

AGRICULTURAL BIOTECHNOLOGY CENTRE

Hungary

Address

Szent Gyorgyi A 4
2100 Godollo

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS

Spain

Address

Campus De La Universidad Autonoma De Madrid
28049 Madrid

FOUNDATION OF RESEARCH AND TECHNOLOGY - HELLAS

Greece

Address

Vassilika Vouton
71110 Iraklion

KEYGENE NV

Netherlands

Address

90, 90 Agro Business Park
6700 AE Wageningen

THE PROVOST, FELLOWS AND SCHOLARS OF THE COLLEGE OF THE HOLY AND UNDIVIDED TRINITY OF QUEEN ELIZABETH NEAR DUBLIN HEREINAFTER TRINITY COLLEGE DUBLIN

Ireland

Address

Smurfit Institute,trinity College
2 Dublin

THE SAINSBURY LABORATORY

United Kingdom

Address

Norwich Research Park, Colney Lane
NR4 7UH Norwich

WAGENINGEN UNIVERSITY

Netherlands

Address

Binnenhaven, 11
6709 PD Wageningen