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Arabidopsis genomic RNA interference knock-out line analysis

Objective

The AGRIKOLA project aims to create a set of 50000 plasmids for performing constitutive or inducible targeted gene-silencing using RNA interference (RNAi) on almost every Arabidopsis gene. At least 6000 of these plasmids will be used to transform Arabidopsis plants to obtain lines in which specific genes (covering the whole of chromosome 3) have been Post transcriptionally silenced. Several hundred of these silenced lines will be analysed in detail to prove the usefulness of this approach and to gain information on the function of important Arabidopsis genes. The plasmids and mutant lines generated during the course of research projects. Such silenced lines will be an invaluable resource for determining the function of Arabidopsis genes, and by extrapolation, the function of homologous genes in other organisms.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
Address
Rue Gaston Crémieux 2, Route De Saint Cyr
91057 Evry
France

Participants (5)

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Spain
Address
115,Campus Universidad Autonoma
28049 Madrid
FLANDERS INTERUNIVERSITY INSTITUTE FOR BIOTECHNOLOGY VZW
Belgium
Address
Ledeganckstraat 35
9000 Gent
HORTICULTURE RESEARCH INTERNATIONAL
United Kingdom
Address

CV35 9EF Wellesbourne, Warwick
IMPERIAL COLLEGE OF SCIENCE, TECHNOLOGY AND MEDICINE
United Kingdom
Address
High Street
TN25 5AH Ashford
UNIVERSITY OF POTSDAM
Germany
Address
10,Maulbeerallee 2
14469 Potsdam