The AGRIKOLA project aims to create a set of 50000 plasmids for performing constitutive or inducible targeted gene-silencing using RNA interference (RNAi) on almost every Arabidopsis gene. At least 6000 of these plasmids will be used to transform Arabidopsis plants to obtain lines in which specific genes (covering the whole of chromosome 3) have been Post transcriptionally silenced. Several hundred of these silenced lines will be analysed in detail to prove the usefulness of this approach and to gain information on the function of important Arabidopsis genes. The plasmids and mutant lines generated during the course of research projects. Such silenced lines will be an invaluable resource for determining the function of Arabidopsis genes, and by extrapolation, the function of homologous genes in other organisms.
Funding SchemeCSC - Cost-sharing contracts
CV35 9EF Wellesbourne, Warwick
TN25 5AH Ashford