Objective We aim to determine the 3D structure of the prototypic neurotransmitter receptor, the nicotinic acetylcholinereceptor (Ache), at high resolution, for understanding the structural basis of its function, using advanced electron and X-ray crystallography, NMR, AFM, molecular modelling and electro physiology, combined with antibody and legend labelling. The best available image of a neurotransmitter receptor is that of the Ache at 4.6AA resolution, obtained by partner 2. Our objectives are to achieve:a. high resolution structure of closed and open Ache channels by electron microscopy;b. imaging individual Aches in action by AFM;c. X-ray structure of Ache channels;d. conformation (by NMR and X-ray crystallography) of Ache fragments;e. characterization of legend and protein binding properties;f. detailed models of Torpedo and human Aches byte combined results, rational design of subtype-specific legends and legend assessment by electro physiology. Elucidation of the structure-function relationship of Aches will be catalytic to numerous studies in synapse function and dysfunction. Fields of science natural sciencesearth and related environmental sciencesgeologymineralogycrystallographynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesphysical sciencesopticsmicroscopyelectron microscopymedical and health sciencesbasic medicinephysiology Programme(s) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Topic(s) 1.1.1.-9. - Neurosciences Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator HELLENIC PASTEUR INSTITUTE Address 127,vassilissis sofias av. 127 11521 Athens Greece See on map EU contribution € 0,00 Participants (6) Sort alphabetically Sort by EU Contribution Expand all Collapse all CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE France EU contribution € 0,00 Address Rue grandville 1 54001 Nancy See on map ELI LILLY COMPANY LIMITED GB EU contribution € 0,00 Address Erl wood manor GU20 6PH Windlesham See on map FREIE UNIVERSITAET BERLIN Germany EU contribution € 0,00 Address Thielallee 63 14195 Berlin See on map MRC Laboratory of Molecular Biology GB EU contribution € 0,00 Address Hills road CB2 2QH Cambridge See on map UNIVERSITAET KONSTANZ Germany EU contribution € 0,00 Address 10,universitaetsstrasse 10 78457 Konstanz See on map UNIVERSITE DE BORDEAUX I France EU contribution € 0,00 Address Avenue pey-berland 16 33607 Pessac See on map
