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Cellular and molecular mechanisms of neuronal retrograde signaling (RETROGRADE SIGNALING)

Objective

In addition to the classical, polarised release of neurotransmitter that takes place at synapses, neurones communicate with each other through another, and less well understood mechanism called retrograde inhibition. Here neurones emit a messenger molecule from their somatodendritic membrane when they fire action potentials, and this molecule induces an inhibitory signal in the axon and synaptic terminals ofpresynaptic neurones. The aim of this proposal is to test whether retrograde inhibition results from the exocytotic release of classical neurotransmitters from the somatodendritic domain of the releasing neuron, and to compare this type ofexocytosis with that taking place at axon terminals. The participation of novelexocytotic proteins, which are insensitive to clostridia neurotoxins, will be investigated. Methods will include molecular biology, immunohistochemistry, electron microscopy, patch-clamp recording and amperommetry.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
Address
Rue Des Saints Pères 45 - Université René Descarte
75270 Paris
France

Participants (3)

INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE
France
Address
Rue De Tolbiac 101
75654 Paris
KAROLINSKA INSTITUTE
Sweden
Address
12,Berzelius Vaeg 3
171 77 Stockholm
UNIVERSITE DE PARIS V 'RENÉ DESCARTES'
France
Address
Rue Des Saints Pères 45
75270 Paris