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Validation and standardisation of diagnostic polymerase chain reaction for detection of food-borne pathogens (FOOD-PCR)

Objective

TECHNOLOGICAL PROBLEM

Recognising that sensitive and more cost-effective methods are needed for detecting food-borne pathogens, we are launching a project seeking to validate and standardise use of the polymerase chain reaction (PCR) for this purpose. Although a powerful research tool, the application of PCR for detecting food-borne pathogens is hampered from lack of validation, standard protocols, reagents, and equipment. Additional specific project objectives include validating a simple method for purifying DNA from bacterial cultures, establishing a central collection of certified DNA sample materials, establishing a databank containing key food-pathogen DNA sequence, listing strains for specificity testing, developing standardised reagents, and validating pre-PCR sample treatment methods.

EXPECTED RESULTS

To facilitate its routine use for diagnostic purposes, participants in the new project are being asked to undertake a series of specific projects, including to construct a DNA sample library and primer databank, validate widely used thermocyclers and other automated equipment, and to develop uniform guidelines describing how tests should be conducted. Because much of the challenge in applying PCR to food-borne pathogens is technical, there is also a need to develop standardised training manuals and procedures for those who will conduct such tests.

APPLICATIONS

The program is planned in 2 phases over a 3-year period and will include 6 work packages and 20 tasks. During phase 1, researchers working in 12 separate laboratories will develop data about the basic methods and requisite reagents. During phase 2, they will focus on validation trials, automation issues, and transferring the validated technology to end-users.

As part of this project, 35 participants are being asked to focus on 5 major pathogens--Salmonella, Campylobactor, enterohemorrhagic E. coli (EHEC), L. monocytogenes and Y. enterocolitica, and on three sample types: poultry carcass-rinse, pig carcass swab, and milk. An expert laboratory is assigned to each pathogen, with the task to prepare defined DNA material, compare the methods and devise and optimise a PCR detection protocol.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

DANISH INSTITUTE FOR FOOD AND VETERINARY RESEARCH
Address
Bulowsvej 27
1790 Koebenhavn V
Denmark

Participants (32)

AGENCE FRANCAISE DE SECURITE SANITAIRE DES ALIMENTS
France
Address
Avenue Du General Leclec 27/31
94701 Maisons-alfort
AGRICULTURAL RESEARCH CENTRE-GENT
Belgium
Address
Brusselsesteenweg 370
9090 Melle, Gontrode
BIOTECON DIAGNOSTICS GMBH
Germany
Address
Hermannswerder Haus 17
14473 Gross Glienicke Potsdam
COMMISSION OF THE EUROPEAN COMMUNITIES
Belgium
Address
Retieseweg
2440 Geel
CONGEN BIOTECHNOLOGIE GMBH
Germany
Address
Robert Roessle Str. 10
Berlin
COOP ITALIA
Italy
Address
Via Del Lavoro, 6/8
40033 Cantagallo
DANISH MEAT RESEARCH INSTITUTE
Denmark
Address
Maglegaardsvej 2
4000 Roskilde
DEPARTMENT FOR ENVIRONMENT, FOOD AND RURAL AFFAIRS
United Kingdom
Address
Sand Hutton
YO41 1LZ York
DIFFCHAMB AB
Sweden
Address
Backa Bergogata 7
Hisings Backa 42246
ESTONIAN AGROBIOCENTRE
Estonia
Address
Roomu Tee 10
51013 Tartu
FEDERAL INSTITUTE FOR HEALTH PROTECTION OF CONSUMERS AND VETERINARY MEDICINE
Germany
Address
Diedersdorfer Weg 1
12277 Berlin
FOOD RESEARCH INSTITUTE
Slovakia
Address
4,Priemyselna 4
820 06 Bratislava
Head of the Unit of Foodborne Zoonoses and Veterinary Epidemiology
Italy
Address
Viale Regina Elena 299
00161 Rome
INSPECTORATE FOR HEALTH PROTECTION, COMMODITIES AND VETERINARY PUBLIC HEALTH
Netherlands
Address
Parnassusplein 5
2500 BC Den Haag ('S-gravenhage)
INSTITUTE OF ANIMAL PHYSIOLOGY - SLOVAK ACADEMY OF SCIENCES
Slovakia
Address
Soltesovej 4
040 01 Kosice
INSTITUTE OF CHEMICAL TECHNOLOGY PRAGUE
Czechia
Address
5,Technicka¡ 5
16628 Praha 6
INSTITUTO DE RECERCA I TECNOLOGIA AGROALIMENTARIES
Spain
Address
Passeig De Gracia 44, 3Pl
08007 Barcelona
JUSTUS-LIEBIG-UNIVERSITY OF GIESSEN
Germany
Address
Frankfurterstrasse 87
35392 Giessen
LUND UNIVERSITY
Sweden
Address
Paradisgatan 2
221 00 Lund
MATFORSK - NORWEGIAN FOOD RESEARCH INSTITUTE
Norway
Address
Osloveien 1
1430 Aas
MEDICANALYSIS LTD
Greece
Address
Fleming 18 Maroysi
15126 Athens
NATIONAL AND KAPODISTRIAN UNIVERSITY OF ATHENS
Greece
Address
Christou Lada Street 6
10561 Athens
NATIONAL INSTITUTE OF PUBLIC HEALTH
Czechia
Address
Palackeho 1-3
612 42 Brno
NATIONAL LABORATORY FOR ENGINEERING AND INDUSTRIAL TECHNOLOGY
Portugal
Address
Estrada Do Paco Do Lumiar 22
1649-038 Lisboa
NATIONAL VETERINARY AND FOOD RESEARCH INSTITUTE
Finland
Address
Hsmeentie 57
00231 Helsinki
NATIONAL VETERINARY RESEARCH INSTITUTE
Poland
Address
Partyznatow 57
24 100 Pulawy
NESTEC S.A.
Switzerland
Address
Avenue Nestle 55
1800 Vevey
QUINTESSENCE RESEARCH AB
Sweden
Address
Malarvagen 9
746 93 Baalsta
TEAGASC - AGRICULTURE AND FOOD DEVELOPMENT AUTHORITY
Ireland
Address
19,Sandymount Avenue 19, Ballsbridge
4 Dublin
UNIVERSIDAD COMPLUTENSE DE MADRID
Spain
Address
2,Avenida De Seneca 2, Ciudad Universitaria
28040 Madrid
UNIVERSITE DE LIEGE*ULG
Belgium
Address
Place Du 20 Aout 7
4000 Liege
UNIVERSITY OF VETERINARY MEDICINE VIENNA
Austria
Address
Veterinaerplatz 1
1210 Wien