The proposal is aimed at understanding the functional interactions between food, intestinal micro flora and health. Novel methods are to be developed to understand and exploit the nutrition driven impact of the human gut micro flora on health. Since the presently used methods for micro flora analysis are time consuming and tedious, high throughput methods for the automated detection of fluorescentlylabelled cells based on microscopic image analysis, flow cytometry and DNA arrays will be developed. In site detection methods for monitoring bacterial gene expression at the cellular level will be developed to monitor the impact of dietary constituents on the transcription of bacterial genes. Samples from human populations will be analysed with the developed methods to identify key factors of micro flora development, composition and activity.
Funding SchemeCSC - Cost-sharing contracts
9713 GX Groningen
RG6 6AP Reading
6703 CT Wageningen