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Development and application of high throughput molecular methods for studying the human gut microbiota in relation to diet and health

Objective

The proposal is aimed at understanding the functional interactions between food, intestinal micro flora and health. Novel methods are to be developed to understand and exploit the nutrition driven impact of the human gut micro flora on health. Since the presently used methods for micro flora analysis are time consuming and tedious, high throughput methods for the automated detection of fluorescentlylabelled cells based on microscopic image analysis, flow cytometry and DNA arrays will be developed. In site detection methods for monitoring bacterial gene expression at the cellular level will be developed to monitor the impact of dietary constituents on the transcription of bacterial genes. Samples from human populations will be analysed with the developed methods to identify key factors of micro flora development, composition and activity.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

GERMAN INSTITUTE OF HUMAN NUTRITION
Address
114-116,Arthur-scheunert-allee 114-116
14558 Bergholz-rehbruecke
Germany

Participants (6)

BIOTECHNOLOGICAL INSTITUTE
Denmark
Address
Kogle Allee 2
2970 Hoersholm
CARL ZEISS VISION GMBH
Germany
Address
Zeppelinstrasse 4
85399 Hallbergmoos
INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
France
Address
Domaine De Vilvert, Bètiment 440
78352 Jouy-en-josas
MICROSCREEN BV
Netherlands
Address
L.j. Zielstraweg 1
9713 GX Groningen
UNIVERSITY OF READING
United Kingdom
Address
Early Gate 4, Whiteknights
RG6 6AP Reading
WAGENINGEN UNIVERSITY
Netherlands
Address
Hesselink Van Suchtelenweg 4
6703 CT Wageningen