Protein complex and target gene purification by in vivo biotinylation

Objective

Novel methodology for the specific in vivo biotinylation of tagged proteins has been developed. It will be used in one-step affinity purification of haematopoietic transcription factor complexes and target genes by streptavidin Chip pull-downs.

Programme(s)

• FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002

Topic(s)

• 1.1.1.-1. - Key action Food, Nutrition and Health

Funding Scheme

RGI - Research grants (individual fellowships)

Coordinator

ERASMUS UNIVERSITEIT ROTTERDAM

Address

Dr. Molewaterplein 50
3000 DR Rotterdam
Netherlands