Vaccines eliciting T cell immunity are likely to be important for protection against diseases such as infection by HIV, HCV or malaria parasites. Current concepts of peptide or protein fragment vaccination do not consider intracellular antigen processing in epitome selection and design of poly-epitome strings. We propose to study proteolysis by immune- and housekeeping protease, and bylysosomal proteases, so as to identify novel epitomes by an enhanced "reverse immunology" approach, to determine distinct epitome sets suitable for preventive and therapeutic vaccines, and to design linkers for optimal intracellular epitoperelease and transport. T cell vaccines for HIV, HCV and malaria will be designed and tested with healthy donor or patient lymphocytes and in a marine malaria model.
Funding SchemeCSC - Cost-sharing contracts
SL5 7PZ Ascot
NW7 1AA London