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Evaluation of lentivirus dna vaccination strategies in sheep

Objective

Lentivirus vaccines have proven difficult to develop to date. The reasons for this include a lack of information on target antigens, protective immune responses, and effective vaccine strategies. We propose to evaluate DNA vaccination strategies in sheep challenged with maedi visna virus (MVV). Sheep will be immunised with MVV core or envelope genes or a combination of these via the skin (by gene gun) or mucosal tissues (using Salmonella vehicles). The effects of including genes encoding IFNg or IL4 during priming will also be investigated. Animals will be boosted with recombinant Adenovirus encoding the MVV genes. Immunological variables as well as virus load and disease will be determined before and after challenge. The project will help to identify protective virus antigens, provide useful information on immune correlates of protection, and indicate whether mucosal or cutaneous immunisation correlates with protection.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

UNIVERSITY OF EDINBURGH
Address
Summerhall
EH9 1QH Edinburgh
United Kingdom

Participants (9)

AGENCE FRANCAISE DE SECURITE SANITAIRE DES ALIMENTS
France
Address
Routes Des Chappes, 105
06902 Sophia Antipolis
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
France
Address
27 Bd Jean Moulin
13385 Marseille
CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Spain
Address
Calle Serrano 117
28006 Madrid
UNIVERSIDAD DE ZARAGOZA
Spain
Address
Miguel Servet 177
50013 Zaragoza
UNIVERSITA DEGLI STUDI DI PISA
Italy
Address
Via Delle Piagge 2
56124 Pisa
UNIVERSITE DE LA MEDITERRANEE D'AIX-MARSEILLE II
France
Address
Boulevard Jean Moulin 27
13385 Marseille
UNIVERSITY OF ICELAND
Iceland
Address
Keldur V/vesturlandsveg
112 Reykjavik
UNIVERSITY OF TORINO
Italy
Address
Via Nizza 52
10126 Torino (Turin)
University of Cambridge
United Kingdom
Address
Madingley Road
CB3 0ES Cambridge