Evaluation of lentivirus dna vaccination strategies in sheep

Objective

Lentivirus vaccines have proven difficult to develop to date. The reasons for this include a lack of information on target antigens, protective immune responses, and effective vaccine strategies. We propose to evaluate DNA vaccination strategies in sheep challenged with maedi visna virus (MVV). Sheep will be immunised with MVV core or envelope genes or a combination of these via the skin (by gene gun) or mucosal tissues (using Salmonella vehicles). The effects of including genes encoding IFNg or IL4 during priming will also be investigated. Animals will be boosted with recombinant Adenovirus encoding the MVV genes. Immunological variables as well as virus load and disease will be determined before and after challenge. The project will help to identify protective virus antigens, provide useful information on immune correlates of protection, and indicate whether mucosal or cutaneous immunisation correlates with protection.

Fields of science

• medical and health sciences › basic medicine › immunology › immunisation
• natural sciences › biological sciences › microbiology › virology
• natural sciences › biological sciences › genetics › DNA
• medical and health sciences › basic medicine › pharmacology and pharmacy › pharmaceutical drugs › vaccines

Programme(s)

• FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002

Topic(s)

• 1.1.1.-2. - Key action Control of Infectious Diseases

Call for proposal

Data not available

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

UNIVERSITY OF EDINBURGH

Address

Summerhall
EH9 1QH Edinburgh
United Kingdom

Participants (9)

AGENCE FRANCAISE DE SECURITE SANITAIRE DES ALIMENTS

France

Address

Routes Des Chappes, 105
06902 Sophia Antipolis

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

France

Address

27 Bd Jean Moulin
13385 Marseille

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS

Spain

Address

Calle Serrano 117
28006 Madrid

UNIVERSIDAD DE ZARAGOZA

Spain

Address

Miguel Servet 177
50013 Zaragoza

UNIVERSITA DEGLI STUDI DI PISA

Italy

Address

Via Delle Piagge 2
56124 Pisa

UNIVERSITE DE LA MEDITERRANEE D'AIX-MARSEILLE II

France

Address

Boulevard Jean Moulin 27
13385 Marseille

UNIVERSITY OF ICELAND

Iceland

Address

Keldur V/vesturlandsveg
112 Reykjavik

UNIVERSITY OF TORINO

Italy

Address

Via Nizza 52
10126 Torino (Turin)

University of Cambridge

United Kingdom

Address

Madingley Road
CB3 0ES Cambridge