Mechanism-guided in vitro evolution

Objective

Using a directed evolution approach, the activities of two structurally similar enzymes, alkaline phosphates and arylsulfatase, will be evolved into each other. The detailed mechanistic characterisation of the new enzymes will address questions about the initial stages of an evolutionary process and the nature of catalysis.

Programme(s)

• FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002

Topic(s)

• 1.1.1.-2. - Key action Control of Infectious Diseases

Funding Scheme

Coordinator