Engineering of the cellular quality control systems in bacillus subtilis for the production of high value-added proteins

Objective

The Bacillus protein secretion machine contains cellular quality control systems that efficiently remove misfolded or incompletely synthesised proteins. Paradoxically, these quality control systems represent a major problem for the production of heterologous proteins at commercially significant concentrations. The ultimate objective is to develop a set of super-producer strains. Full use will be made of recent advances in genomics (eg.: DNA arrays), proteomics, bioinformatics, directed molecular evolution and information obtained from previous, EU-funded programmes on Bacillus protein secretion, genome sequencing and the systematic gene fuction analysis. The proposal includes the potential to 'spin-off a research-based biotechnology SME or trust company.

Programme(s)

• FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002

Topic(s)

• 1.1.1.-3. - Key action The "Cell factory"

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

NETHERLANDS RESEARCH SCHOOL FOR ASTRONOMY - LEGALLY REPRESENTED BY UNIVERSITY OF GRONINGEN

Address

5,Broerstraat 5
9712 CP Groningen
Netherlands

Participants (9)

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

France

Address

Place Jussieu 2, Tour 43
75251 Paris

FORSCHUNGSZENTRUM JUELICH GMBH

Germany

Address

Leo-brand-strasse
52425 Juelich

GENENCOR INTERNATIONAL BV

Netherlands

Address

1,Archimedesweg 30
2333 Leiden

NATIONAL PUBLIC HEALTH INSTITUTE

Finland

Address

Mannerheimintie 166
Helsinki

NOVOZYMES A/S

Denmark

Address

Kroegshoejvej 36
2880 Bagsvaerd

UNIVERSITAET BAYREUTH

Germany

Address

Universitaetsstrasse 30
95447 Bayreuth

UNIVERSITY OF GREIFSWALD

Germany

Address

F.-l.-jahnstrasse 15
17487 Greifswald

UNIVERSITY OF NEWCASTLE UPON TYNE

United Kingdom

Address

NE1 7RU Newcastle Upon Tyne

UNIVERSITÉ DE PARIS VII DENIS DIDEROT

France

Address

Place De Jussieu 2 Tour 43
75251 Paris