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Engineering of the cellular quality control systems in bacillus subtilis for the production of high value-added proteins

Objective

The Bacillus protein secretion machine contains cellular quality control systems that efficiently remove misfolded or incompletely synthesised proteins. Paradoxically, these quality control systems represent a major problem for the production of heterologous proteins at commercially significant concentrations. The ultimate objective is to develop a set of super-producer strains. Full use will be made of recent advances in genomics (eg.: DNA arrays), proteomics, bioinformatics, directed molecular evolution and information obtained from previous, EU-funded programmes on Bacillus protein secretion, genome sequencing and the systematic gene fuction analysis. The proposal includes the potential to 'spin-off a research-based biotechnology SME or trust company.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

NETHERLANDS RESEARCH SCHOOL FOR ASTRONOMY - LEGALLY REPRESENTED BY UNIVERSITY OF GRONINGEN
Address
5,Broerstraat 5
9712 CP Groningen
Netherlands

Participants (9)

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
France
Address
Place Jussieu 2, Tour 43
75251 Paris
FORSCHUNGSZENTRUM JUELICH GMBH
Germany
Address
Leo-brand-strasse
52425 Juelich
GENENCOR INTERNATIONAL BV
Netherlands
Address
1,Archimedesweg 30
2333 Leiden
NATIONAL PUBLIC HEALTH INSTITUTE
Finland
Address
Mannerheimintie 166
Helsinki
NOVOZYMES A/S
Denmark
Address
Kroegshoejvej 36
2880 Bagsvaerd
UNIVERSITAET BAYREUTH
Germany
Address
Universitaetsstrasse 30
95447 Bayreuth
UNIVERSITY OF GREIFSWALD
Germany
Address
F.-l.-jahnstrasse 15
17487 Greifswald
UNIVERSITY OF NEWCASTLE UPON TYNE
United Kingdom
Address

NE1 7RU Newcastle Upon Tyne
UNIVERSITÉ DE PARIS VII DENIS DIDEROT
France
Address
Place De Jussieu 2 Tour 43
75251 Paris