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The European sulfolobus project. Screening for and expressing thermostable enzymes of industrial interest from sulfolobus and other crenarchaeotes

Objective

The Crenarchaeotes constitute a kingdom of the Archaea that contains a very diverse range of microorganisms many of which are extreme- or hyperthermophiles. They differ extensively genetically and biochemically from Bacteria and Eukaryotes. Sulfolobus is the best studied of these organisms and most of ground-breaking work on this organism has been done in Europe. The purpose of this project is to exploit this European advantage and to screen for novel enzyme activities both at the genomic level and in an extensive and unique European collection of thermophilic Crenarchaeotes. Moreover, we will develop and refine vector systems for expressing these proteins in a range of Bacterial and Eukaryotic hosts by modifying the Archaeal genetic signals. In particular, we will develop, for the first time, a vector-host system for expressing Crenarchaeal enzymes in Sulfolobus on a large scale.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

UNIVERSITY OF COPENHAGEN
Address
Oester Voldgade 3
1350 Koebenhavn K/copenhaegen
Denmark

Participants (8)

EPIDAUROS BIOTECHNOLOGIE AG
Germany
Address
Am Neuland 1
82347 Bernried
MICROBIOLOGICAL RESEARCH AUTHORITY
United Kingdom
Address
Porton Down
SP4 0JG Salisbury
NOVOZYMES A/S
Denmark
Address
Kroegshoejvej 36
2880 Bagsvaerd
TECHNICAL UNIVERSITY OF DENMARK
Denmark
Address
Anker Engelundsvej 1, Building 101
2800 Lyngby
UNIVERSITA DEGLI STUDI DI NAPOLI FEDERICO II
Italy
Address
Via Mezzocannone 16
80134 Napoli
UNIVERSITAET DARMSTADT/TU
Germany
Address
Schnittspahnstr. 10
64287 Darmstadt
UPPSALA UNIVERSITY
Sweden
Address
Sankt Olofsgatan 10 B
75105 Uppsala
WAGENINGEN UNIVERSITY
Netherlands
Address
Bomenweg 2
6700 EV Wageningen