The Crenarchaeotes constitute a kingdom of the Archaea that contains a very diverse range of microorganisms many of which are extreme- or hyperthermophiles. They differ extensively genetically and biochemically from Bacteria and Eukaryotes. Sulfolobus is the best studied of these organisms and most of ground-breaking work on this organism has been done in Europe. The purpose of this project is to exploit this European advantage and to screen for novel enzyme activities both at the genomic level and in an extensive and unique European collection of thermophilic Crenarchaeotes. Moreover, we will develop and refine vector systems for expressing these proteins in a range of Bacterial and Eukaryotic hosts by modifying the Archaeal genetic signals. In particular, we will develop, for the first time, a vector-host system for expressing Crenarchaeal enzymes in Sulfolobus on a large scale.
Funding SchemeCSC - Cost-sharing contracts
SP4 0JG Salisbury
6700 EV Wageningen