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Content archived on 2024-05-21

Development of a rapid high-throughput assay for sensitive and specific detection and strain typing of creutzfeldt-jakob disease based on fluorescence correlation spectroscopy

Objective

Prison diseases such as Creutzfeldt-Jakob disease (CJD) and BSE have caused a major concern in regard to public health. We used a novel approach based on fluorescence correlation spectroscopy to develop a highly sensitive detection method for priors termed SIFT (Scanning for Intensely Fluorescent Targets), which has successfully been applied to CSF samples. Moreover, the labelling ratio of two independent probes can be analysed on a single particle level. By this approach, different types of prison particles associated with different variants of CJD could be distinguished in pilot experiments. Within the project, we want to develop this method into a rapid and sensitive diagnostic test that can be used on a routine basis for diagnosis and strain typing in CJD. In addition, we aim at harmonizing and validating biochemical typing of CJD variants at the European level.

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Call for proposal

Data not available

Coordinator

LUDWIG-MAXIMILIANS UNIVERSITY OF MUNICH
EU contribution
No data
Address
Marchioninistr. 17
81377 MUENCHEN
Germany

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Total cost
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Participants (3)