Target specific delivery systems for gene therapy based on cell penetrating peptides

Objective

The cell membrane penetrating peptides, Capps, are designed and synthesized recently by the partners, and applied for cellular delivery of oligonucleotides. The aim of the present project is the design, synthesis and testing of novel on-viral modes of efficient target selective transport vectors based on CPPstrategy for delivery of DNA single and double strand cargos and applications ingénue therapy. For that, the mechanisms of cell penetration of Capps will be studied. Specific tissue, cell and sub cellular compartment targeting Capps will be developed for application in gene therapy. The libraries of Capps will besynthesised and tested with combination of iterative approach and high throughput screening. The biological effects of successful oligonucleotide delivery systems will be detected in vitro and in vivo, and used in a high-throughput assay of CPPoptimisation

Fields of science

• medical and health sciences › medical biotechnology › genetic engineering › gene therapy
• natural sciences › biological sciences › genetics › DNA
• natural sciences › biological sciences › biochemistry › biomolecules

Programme(s)

• FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002

Topic(s)

• 1.1.1.-3. - Key action The "Cell factory"

Call for proposal

Data not available

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

STOCKHOLM UNIVERSITET

Address

Universitetsvaegen 10
10691 Stockholm
Sweden

Participants (9)

ASTRAZENECA UK LTD

United Kingdom

Address

Stanhope Gate 15
W1Y 6LN London

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

France

Address

Rue Michel-ange 3
75794 Paris

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

France

Address

46,rue D' Ulm 46 Ecole Normale Superieure
75230 Paris

CEPEP AB

Sweden

Address

S. Arrheniusvaegen
10891 Stockholm

ECOLE NORMALE SUPERIEURE

France

Address

Rue D'ulm 46, Ecole Normale Supérieure
75230 Paris

ESTONIAN BIOCENTRE

Estonia

Address

Riia St. 23
51010 Tartu

FORSCHUNGSVERBUND BERLIN E.V.

Germany

Address

Rudower Chaussee 17
12489 Berlin

Medical Research Council (MRC)

United Kingdom

Address

20 Park Crescent
W1N 4AL London

UNIVERSITE DE MONTPELLIER II - SCIENCES ET TECHNIQUES DU LANGUEDOC

France

Address

Route De Mende, 1919
34293 Montpellier