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Isolation of regulatory elements controlling the formation of isoprenoids

Objective

Objective To isolate from tomato regulatory gene sequences that control isoprene formation in fruit that control isoprene formation in fruit. This will be achieved by:
1. Preparation of an activation tang T -DNA vector containing Cam 35 S enhancers and phone syntheses promoter
2. Transformation of the Micro tom variety of tomato with the T DNA vectors and tissue culture
3. Regeneration of transgenic plants and phenotypic analysis by metabolite profiling of isoprenoids
4. Detailed analysis of fruit for altered carotenoid/isoprenoid changes
5. Characterisation of T DNA insertions of plants with altered isoprene levels
6. Generation of stable progeny and confirmation of phenotype by retransformation of tagged sequences

Coordinator

ROYAL HOLLOWAY AND BEDFORD NEW COLLEGE
Address
Egham Hill
TW20 0EX Egham,surrey
United Kingdom