Objective
The sea bass is a major farmed fish in the EU but, as happens for other species, still presents unpredictable and unsynchronized spawns in captivity. The process of reproduction is controlled by the pituitary gonadotropins (GtHs), which release is regulated from the brain by the GonadotropinReleasing Hormone (QnRH). Our knowledge of the hormonal mechanisms of spawning are very poor in sea bass. Attempts to induce spawning have used a limited number of GnRH analogues (GnRHa) and have never considered the availability of pituitary GnRH receptors (GnRH-R), as determinants of gonadotroph sensitivity to the GnRH stimulus. The present project aims at investigating the GnRH system in sea bass, following molecular and physiological approaches, with special emphasis on the GrIRH-R. The GnRH-R will be cloned and distribution of mRNA studied by RT-PCR. The seasonal expression of GnRH-R, as well as sbGrIRH, sGnRH and cGnR11-11 mRNAs will be monitored, by developing specific dot-blot techniques, which will provide information on, the physiological relevance of each native GnRH and the moment of maximal gonadotroph sensitivity. On another hand, the GnRH-R cDNA will be stable expressed in HEK 293 cells and a culture system set up to perform studies on GrIRH-R binding potency of new GrIRHa; these data will be correlated with the biological activities of these GrIRHa in vivo (GTH-2 releasing potency and spawning induction capacity). We expect that the knowledge and tools obtained from this work, will help to the development of more effective spawning induction therapies for the aquaculture industry, based on the determination of the optimal physiological stage of the fish for hormonal treatment and the use of new GnRHa.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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Coordinator
28006 Madrid
Spain
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