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Content archived on 2024-05-24

Investigating the role of rs2-like genes during leaf initiation

Objective

The objective of this proposal is to further investigate the role of rough sheath 2 (rest) -like genes during leaf development in Zeal Mays and Arabidopsis thalami. The proposed work should increase our knowledge of vegetative development in general and developmental switches in particular. The project has three main objectives: New maize mutants with phenotypes similar to rest are being- identified in Spam mutagenized populations. I aim to characterize one such mutant, corkscrew (cakes) and to clone the mutated gene. Using the reverse approach, I aim to clone a like-like gene (Zoril) that has been identified by low stringency hybridisation with like. Insert ional mutants will subsequently be identified in Mud-. Mutagenized populations by PCR. The third objective is to identify genes, which respond to RS2 action. This will be carried out using like mutant maize plants and transgenic Arabidopsis plants over expressing the rs2-ortholog; asymmetric leaves 1 (as). RNA isolated from relevant and specific tissues, namely P3 leaf- primordial and apical moistens respectively will be analysed by micro array analysis. Comparisons between wild type and mutant (transformed) plants should identify genes, which are regulated by like-like genes. These genes will subsequently be characterised. Information obtained from all three approaches will be used to determine how like, cakes, Zoril and Knox genes interact to facilitate the initiation and development of lateral organs.

Fields of science (EuroSciVoc)

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Topic(s)

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Call for proposal

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Funding Scheme

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RGI - Research grants (individual fellowships)

Coordinator

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
EU contribution
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Address
SOUTH PARKS ROAD
OX1 3RB OXFORD
United Kingdom

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Total cost

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