Enzyme discovery in hybrid aspen for fibre engineering

Objective

The main aim is enzyme discovery from 8000 hybrid aspen EST-sequences for their eventual use in fibre engineering. The stepwise selection scheme includes:
1) Identification of genes highly expressed during wood formation using the micro array technology for reran expression profiling,
2) Novel bio informatics software for sensitive analysis of distant evolutionary relationships and modular structures of selected enzymes,
3) Heterogonous expression and enzymatic characterization of selected enzymes,
4) Fibre analysis of Arabidopsis mutants in orthologous genes by reverse genetics,
5) Protein expression, localization and fibre analyses in transgenic aspen with up/Dow regulated expression of selected genes,
6) Micro pulping of selected fibre samples to correlate the enzymatic activities with final product properties.

Fields of science

• natural sciences › biological sciences › genetics
• natural sciences › computer and information sciences › software
• natural sciences › biological sciences › biochemistry › biomolecules › proteins › enzymes
• agricultural sciences › agriculture, forestry, and fisheries › forestry

Programme(s)

• FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002

Topic(s)

• 1.1.1.-5. - Key action Sustainable Agriculture, Fisheries and Forestry

Call for proposal

Data not available

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

ROYAL INSTITUTE OF TECHNOLOGY

Address

79,valhallavaegen 79
10044 Stockholm
Sweden

Participants (7)

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

France

Address

31,chemin Joseph Aiguier 31
13402 Marseille

FLANDERS INTERUNIVERSITY INSTITUTE FOR BIOTECHNOLOGY VZW

Belgium

Address

Ledeganckstraat 35
9000 Gent

JOHN INNES CENTRE

United Kingdom

Address

Colney Lane - Norwich Research Park
NR4 7UH Norwich

STFI-PACKFORSK AB

Sweden

Address

Drottning Kristinas Vaeg 61
Stockholm

Links

THE SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES

Sweden

Address

Goesta Skoglund St
901 83 Umeaa

UNIVERSITAT RAMON LLULL

Spain

Address

Via Augusta 390
08017 Barcelona

UNIVERSITY OF NOTTINGHAM

United Kingdom

Address

Sutton Bonington Campus
LE12 5RD Loughborough