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Enzyme discovery in hybrid aspen for fibre engineering

Objective

The main aim is enzyme discovery from 8000 hybrid aspen EST-sequences for their eventual use in fibre engineering. The stepwise selection scheme includes:
1) Identification of genes highly expressed during wood formation using the micro array technology for reran expression profiling,
2) Novel bio informatics software for sensitive analysis of distant evolutionary relationships and modular structures of selected enzymes,
3) Heterogonous expression and enzymatic characterization of selected enzymes,
4) Fibre analysis of Arabidopsis mutants in orthologous genes by reverse genetics,
5) Protein expression, localization and fibre analyses in transgenic aspen with up/Dow regulated expression of selected genes,
6) Micro pulping of selected fibre samples to correlate the enzymatic activities with final product properties.

Call for proposal

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Coordinator

ROYAL INSTITUTE OF TECHNOLOGY
EU contribution
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Address
79,Valhallavaegen 79
10044 STOCKHOLM
Sweden

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Total cost
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Participants (7)