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Enzyme discovery in hybrid aspen for fibre engineering

Objective

The main aim is enzyme discovery from 8000 hybrid aspen EST-sequences for their eventual use in fibre engineering. The stepwise selection scheme includes:
1) Identification of genes highly expressed during wood formation using the micro array technology for reran expression profiling,
2) Novel bio informatics software for sensitive analysis of distant evolutionary relationships and modular structures of selected enzymes,
3) Heterogonous expression and enzymatic characterization of selected enzymes,
4) Fibre analysis of Arabidopsis mutants in orthologous genes by reverse genetics,
5) Protein expression, localization and fibre analyses in transgenic aspen with up/Dow regulated expression of selected genes,
6) Micro pulping of selected fibre samples to correlate the enzymatic activities with final product properties.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

ROYAL INSTITUTE OF TECHNOLOGY
Address
79,Valhallavaegen 79
10044 Stockholm
Sweden

Participants (7)

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
France
Address
31,Chemin Joseph Aiguier 31
13402 Marseille
FLANDERS INTERUNIVERSITY INSTITUTE FOR BIOTECHNOLOGY VZW
Belgium
Address
Ledeganckstraat 35
9000 Gent
JOHN INNES CENTRE
United Kingdom
Address
Colney Lane - Norwich Research Park
NR4 7UH Norwich
STFI-PACKFORSK AB
Sweden
Address
Drottning Kristinas Vaeg 61
Stockholm
THE SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES
Sweden
Address
Goesta Skoglund St
901 83 Umeaa
UNIVERSITAT RAMON LLULL
Spain
Address
Via Augusta 390
08017 Barcelona
UNIVERSITY OF NOTTINGHAM
United Kingdom
Address
Sutton Bonington Campus
LE12 5RD Loughborough