In the last few years, there has been a surge of interest in the use of light microscopy in biomedical research, driven by the increasing need of biologists, in the post-genomic era, to map chemical and molecular activities in living systems. This has been made approachable by recent advances in the fields of biology, chemistry, physical optics, and robotics and computer science. This combination of technologies promises to advance research, create new tools for biotechnology and clinical applications, and form the basis for new clinical diagnostic tests. However, such a potential is still far from being fully exploited. European laboratories form EAMNET with expertise in research in advanced light microscopy applied to all fields of modern biology. Its main goal is to make the potential of modern light microscopy available to all the interested European laboratories, with an emphasis on both pre-clinical research and on pharmaceutical applications.