The objective of the proposed work is to develop advanced genomic tools that will help researchers to identify the genes involved in important traits in cattle, starting either from their map position, or from examining expression profiles. A set of 20,000 non-redundant cDNAs will be sequenced. These cDNAS will be used to construct macro-and micro-arrays, which can be used to examine gene expression under various physiological conditions. The sequence information will also be used to construct a high-density "gene map" of the bovine genome and hence high-resolution comparative genome maps with man and mouse. The latter will aid the identification of postional candidate genes for mapped traits. The sequence information and RH maps will also be used to in collaboration with an international project to construct a scaffold physical (BAC) map of the entire bovine genome, which will be as entry for targeted DNA sequencing.
Funding SchemeCSC - Cost-sharing contracts
T6G 2P Edmonton