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CREATINE KINASE

Objective



Creatine kinase (CK) is an enzyme which is present in the cells of most organs. The determination of creatine kinase, and particularly of the three isoenzymes from brain (CK-BB), skeletal muscle (CK-MM) and heart muscle (CK-MB) respectively, is currently performed in clinical chemistry laboratories. The brain isoenzyme (CK-BB) is found elevated in serum of patients with certain neurological diseases.

The project consists of the purification of creatine kinase isoenzyme CK-BB from human placenta. The aim is to certify the catalytic activity concentration of creatine kinase in this well characterized purified enzyme preparation. The method used for the assay is that recommended by the International Federation of Clinical Chemistry (IFCC).

RESULTS

The feasibility study could establish that a stable purified enzyme preparation could be produced in lyophilized form. An interlaboratory study was carried out on that preparation with a view to assigning a value to the CK catalytic activity concentration of the reconstituted material. Nine laboratories participated. The agreement between laboratories was judged satisfactory, permitting to propose the preparation for certification with the following value and uncertainty:
CRM 299 (325 +/- 10) U/l.
The report describing the preparation and the certification exercise has been published as report EUR 13886 (1992).

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Hospices Civils de Lyon
Address

Lyon
France

Participants (9)

Boehringer Mannheim GmbH
Germany
Address

Penzberg
Centre Hospitalier Universitaire Vaudois
Switzerland
Address

Lausanne
Centre du Médicament
France
Address

Nancy
Medizinische Universität Lübeck
Germany
Address
Ratzeburger Allee 160
23562 Lübeck
Metaxas Memorial Cancer Hospital
Greece
Address

Piraeus
National Institute for Biological Standards and Control (NIBSC)
United Kingdom
Address
Blanche Lane South Mimms
EN6 3QG Potters Bar
Odense University Hospital
Denmark
St Radboud Ziekenhuis
Netherlands
Address

Nijmegen
UNIVERSIDAD AUTONOMA DE BARCELONA
Spain
Address
Campus Universitari