Exploitable results Determination of hydrogen deuterium (H/D) isotopic ratio at natural abundance level in wine by quantitative nuclear magnetic resonance (NMR) spectrometry The use of nuclear magnetic resonance (NMR) to detect the fraudulent addition of sugar and water to grapes during wine making, to increase the alcoholic strength of wine is well established. The deuterium isotope redistribution from sugar to ethanol during fermentation is characterized by the hydrogen to deuterium H/D ratio but the method requires a set of reference ethanols of known H/D ratio at natural abundance levels for the calibration, and alignment of NMR spectrometers. In the project 3 ethanol reference materials (RM) from different phytobiological origins were certified: ethanol from sugar beet, a Calvin type plant which normally has a low deuterium content; ethanol from grape having a medium deuterium content; ethanol from maize, a Hatch-Slack type plant with high deuterium content. A reference tetramethylurea (TMU) sample for use as an internal standard was also prepared in 500 and 1000 ml quantities for official site specific natural isotope fractionation (SNIF) NMR measurements of wine and must. Searching for OpenAIRE data... There was an error trying to search data from OpenAIRE No results available
